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Addgene inc hdm tat1b
Hdm Tat1b, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hdm+tat1b/RGS-6xHis-BLIMP-1-pcDNA3%2E1-+(Plasmid+%2352518)/pm41053501-251-39-57
Average 93 stars, based on 10 article reviews
hdm tat1b - by Bioz Stars, 2026-09
93/100 stars

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Related Articles

Plasmid Preparation:

Article Title: Direct Cryo-ET observation of platelet deformation induced by SARS-CoV-2 Spike protein
Article Snippet: Necessary reagents for the production were obtained from BEI resources, NIAID, NIH. .. Briefly, X-Lenti 293T cells (Takara #632180) plated in 6-well plates were co-transfected with 1 μg of lentiviral backbone ZsGreen (BEI #NR-52516); 0.22 μg each of the helper plasmids HDM-Hgpm2 (BEI #NR-52517), pRC-CMV-Rev1b (BEI #NR-52519), HDM-tat1b (BEI #NR-52518); and 0.34 μg of viral entry protein (SARS-CoV-2 Spike, BEI #NR-53742) or pCMV-VSVG (a gift from B. Weinberg, Addgene plasmid #8454) using 8 μl of TransIT-293 (Mirus Bio #MIR 2705) transfection reagent per well. ..

Article Title: Novel CRITR-seq approach reveals influenza transcription is modulated by NELF and is a key event precipitating an interferon response
Article Snippet: .. Plasmid libraries were transfected using Lipofectamine 3000 and P3000, along with lentiviral helper plasmids HDM-Hgpm2 (Addgene #204152), HDM-tat1b (Addgene #204154), pRC-CMV-Rev1 (Addgene #164443), and HDM_VSV_G (Addgene #204156), at 10.27 μg divided equally between them. ..

Article Title: Direct Cryo-ET observation of platelet deformation induced by SARS-CoV-2 Spike protein
Article Snippet: Necessary reagents for the production were obtained from BEI resources, NIAID, NIH. .. Briefly, X-Lenti 293T cells (Takara #632180) plated in 6-well plates were co-transfected with 1 μg of lentiviral backbone ZsGreen (BEI #NR-52516); 0.22 μg each of the helper plasmids HDM-Hgpm2 (BEI #NR-52517), pRC-CMV-Rev1b (BEI #NR-52519), HDM-tat1b (BEI #NR-52518); and 0.34 μg of viral entry protein (SARS-CoV-2 Spike, BEI #NR-53742) or pCMV-VSVG (a gift from B. Weinberg, Addgene plasmid #8454) using 8 μl of TransIT-293 (Mirus Bio #MIR 2705) transfection reagent per well. ..

Article Title: CRISPR with Transcriptional Readout reveals influenza transcription is modulated by NELF and can precipitate an interferon response
Article Snippet: .. Plasmid libraries were transfected using Lipofectamine 3000 and P3000, along with lentiviral helper plasmids HDM-Hgpm2 (Addgene #204152), HDM-tat1b (Addgene #204154), pRC-CMV-Rev1 (Addgene #164443), and HDM_VSV_G (Addgene #204156), at 10.27 μg divided equally between them. .. Plasmid libraries were transfected using Lipofectamine 3000 and P3000, along with lentiviral helper plasmids HDM-Hgpm2 (Addgene #204152), HDM-tat1b (Addgene #204154), pRC-CMV-Rev1 (Addgene #164443), and HDM_VSV_G (Addgene #204156), at 10.27 μg divided equally between them.

Article Title: Development of an ultrahigh affinity, trimeric ACE2 biologic as a universal SARS-CoV-2 antagonist
Article Snippet: Pseudotyped virus particles were produced in HEK293T cells (BEI Resources) as previously described . .. Briefly, HEK293T cells were seeded in a six-well plate (5 × 10 5 per well) and transfected 24 h post-seeding with the following plasmids: 1 μg of lentiviral backbone (NR-52516); 0.22 μg each of plasmids HDM-Hgpm2 (NR-52517), pRC-CMV-Rev1b (NR-52519), and HDM-tat1b (NR-52518); and 0.34 μg viral entry protein for: SARS-CoV-2 Spike Wuhan-Hu-1 (BEI Resources NR-53742), SARS-CoV-2 Delta strain (Addgene plasmid #155130), SARS-CoV-2 Omicron BA.1 (Addgene plasmid #180375), SARS-CoV-2 Arcturus XBB.1.16 (Addgene plasmid #201189), or SARS-CoV-1 WT (Addgene plasmid #170447). ..

Article Title: CRISPR with Transcriptional Readout reveals influenza transcription is modulated by NELF and can precipitate an interferon response
Article Snippet: .. Lentivirus expressing codon-optimized NELF-B was generated by seeding 4x105 HEK293T cells in a 6-well plate with D10, followed by transfection with 1 μg DNA from a plasmid encoding codon-optimized NELFB (sequence provided in Dataset S11), and 1 μg DNA consisting of equal amounts of the lentiviral helper plasmids HDM-Hgpm2 (Addgene #204152), HDM-tat1b (Addgene #204154), pRC-CMV-Rev1 (Addgene #164443), and HDM_VSV_G (Addgene #204156). ..

Article Title: Direct Cryo-ET observation of platelet deformation induced by SARS-CoV-2 spike protein
Article Snippet: Necessary reagents for the production were obtained from BEI resources, NIAID, NIH. .. Briefly, X-Lenti 293T cells (Takara #632180) plated in six-well plates were co-transfected with 1 μg of lentiviral backbone ZsGreen (BEI #NR-52516); 0.22 μg each of the helper plasmids HDM-Hgpm2 (BEI #NR-52517), pRC-CMV-Rev1b (BEI #NR-52519), HDM-tat1b (BEI #NR-52518); and 0.34 μg of viral entry protein (SARS-CoV-2 Spike, BEI #NR-53742) or pCMV-VSVG (a gift from B. Weinberg, Addgene plasmid #8454) using 8 μl of TransIT-293 (Mirus Bio #MIR 2705) transfection reagent per well. ..

Article Title: Development of an ultrahigh affinity, trimeric ACE2 biologic as a universal SARS-CoV-2 antagonist.
Article Snippet: Pseudotyped virus particles were produced in HEK293T cells (BEI Resources) as previously described67. .. Briefly, HEK293T cells were seeded in a six-well plate (5 × 105 per well) and transfected 24 h post-seeding with the following plasmids: 1 μg of lentiviral backbone (NR-52516); 0.22 μg each of plasmids HDM-Hgpm2 (NR-52517), pRC-CMV-Rev1b (NR-52519), and HDM-tat1b (NR-52518); and 0.34 μg viral entry protein for: SARS-CoV-2 Spike Wuhan-Hu-1 (BEI Resources NR-53742), SARS-CoV-2 Delta strain (Addgene plasmid #155130), SARS-CoV-2 Omicron BA.1 (Addgene plasmid #180375), SARS-CoV-2 Arcturus XBB.1.16 (Addgene plasmid #201189), or SARS-CoV-1WT (Addgene plasmid #170447). ..

Transfection:

Article Title: Direct Cryo-ET observation of platelet deformation induced by SARS-CoV-2 Spike protein
Article Snippet: Necessary reagents for the production were obtained from BEI resources, NIAID, NIH. .. Briefly, X-Lenti 293T cells (Takara #632180) plated in 6-well plates were co-transfected with 1 μg of lentiviral backbone ZsGreen (BEI #NR-52516); 0.22 μg each of the helper plasmids HDM-Hgpm2 (BEI #NR-52517), pRC-CMV-Rev1b (BEI #NR-52519), HDM-tat1b (BEI #NR-52518); and 0.34 μg of viral entry protein (SARS-CoV-2 Spike, BEI #NR-53742) or pCMV-VSVG (a gift from B. Weinberg, Addgene plasmid #8454) using 8 μl of TransIT-293 (Mirus Bio #MIR 2705) transfection reagent per well. ..

Article Title: Novel CRITR-seq approach reveals influenza transcription is modulated by NELF and is a key event precipitating an interferon response
Article Snippet: .. Plasmid libraries were transfected using Lipofectamine 3000 and P3000, along with lentiviral helper plasmids HDM-Hgpm2 (Addgene #204152), HDM-tat1b (Addgene #204154), pRC-CMV-Rev1 (Addgene #164443), and HDM_VSV_G (Addgene #204156), at 10.27 μg divided equally between them. ..

Article Title: Direct Cryo-ET observation of platelet deformation induced by SARS-CoV-2 Spike protein
Article Snippet: Necessary reagents for the production were obtained from BEI resources, NIAID, NIH. .. Briefly, X-Lenti 293T cells (Takara #632180) plated in 6-well plates were co-transfected with 1 μg of lentiviral backbone ZsGreen (BEI #NR-52516); 0.22 μg each of the helper plasmids HDM-Hgpm2 (BEI #NR-52517), pRC-CMV-Rev1b (BEI #NR-52519), HDM-tat1b (BEI #NR-52518); and 0.34 μg of viral entry protein (SARS-CoV-2 Spike, BEI #NR-53742) or pCMV-VSVG (a gift from B. Weinberg, Addgene plasmid #8454) using 8 μl of TransIT-293 (Mirus Bio #MIR 2705) transfection reagent per well. ..

Article Title: CRISPR with Transcriptional Readout reveals influenza transcription is modulated by NELF and can precipitate an interferon response
Article Snippet: .. Plasmid libraries were transfected using Lipofectamine 3000 and P3000, along with lentiviral helper plasmids HDM-Hgpm2 (Addgene #204152), HDM-tat1b (Addgene #204154), pRC-CMV-Rev1 (Addgene #164443), and HDM_VSV_G (Addgene #204156), at 10.27 μg divided equally between them. .. Plasmid libraries were transfected using Lipofectamine 3000 and P3000, along with lentiviral helper plasmids HDM-Hgpm2 (Addgene #204152), HDM-tat1b (Addgene #204154), pRC-CMV-Rev1 (Addgene #164443), and HDM_VSV_G (Addgene #204156), at 10.27 μg divided equally between them.

Article Title: Development of an ultrahigh affinity, trimeric ACE2 biologic as a universal SARS-CoV-2 antagonist
Article Snippet: Pseudotyped virus particles were produced in HEK293T cells (BEI Resources) as previously described . .. Briefly, HEK293T cells were seeded in a six-well plate (5 × 10 5 per well) and transfected 24 h post-seeding with the following plasmids: 1 μg of lentiviral backbone (NR-52516); 0.22 μg each of plasmids HDM-Hgpm2 (NR-52517), pRC-CMV-Rev1b (NR-52519), and HDM-tat1b (NR-52518); and 0.34 μg viral entry protein for: SARS-CoV-2 Spike Wuhan-Hu-1 (BEI Resources NR-53742), SARS-CoV-2 Delta strain (Addgene plasmid #155130), SARS-CoV-2 Omicron BA.1 (Addgene plasmid #180375), SARS-CoV-2 Arcturus XBB.1.16 (Addgene plasmid #201189), or SARS-CoV-1 WT (Addgene plasmid #170447). ..

Article Title: CRISPR with Transcriptional Readout reveals influenza transcription is modulated by NELF and can precipitate an interferon response
Article Snippet: .. Lentivirus expressing codon-optimized NELF-B was generated by seeding 4x105 HEK293T cells in a 6-well plate with D10, followed by transfection with 1 μg DNA from a plasmid encoding codon-optimized NELFB (sequence provided in Dataset S11), and 1 μg DNA consisting of equal amounts of the lentiviral helper plasmids HDM-Hgpm2 (Addgene #204152), HDM-tat1b (Addgene #204154), pRC-CMV-Rev1 (Addgene #164443), and HDM_VSV_G (Addgene #204156). ..

Article Title: Direct Cryo-ET observation of platelet deformation induced by SARS-CoV-2 spike protein
Article Snippet: Necessary reagents for the production were obtained from BEI resources, NIAID, NIH. .. Briefly, X-Lenti 293T cells (Takara #632180) plated in six-well plates were co-transfected with 1 μg of lentiviral backbone ZsGreen (BEI #NR-52516); 0.22 μg each of the helper plasmids HDM-Hgpm2 (BEI #NR-52517), pRC-CMV-Rev1b (BEI #NR-52519), HDM-tat1b (BEI #NR-52518); and 0.34 μg of viral entry protein (SARS-CoV-2 Spike, BEI #NR-53742) or pCMV-VSVG (a gift from B. Weinberg, Addgene plasmid #8454) using 8 μl of TransIT-293 (Mirus Bio #MIR 2705) transfection reagent per well. ..

Article Title: Development of an ultrahigh affinity, trimeric ACE2 biologic as a universal SARS-CoV-2 antagonist.
Article Snippet: Pseudotyped virus particles were produced in HEK293T cells (BEI Resources) as previously described67. .. Briefly, HEK293T cells were seeded in a six-well plate (5 × 105 per well) and transfected 24 h post-seeding with the following plasmids: 1 μg of lentiviral backbone (NR-52516); 0.22 μg each of plasmids HDM-Hgpm2 (NR-52517), pRC-CMV-Rev1b (NR-52519), and HDM-tat1b (NR-52518); and 0.34 μg viral entry protein for: SARS-CoV-2 Spike Wuhan-Hu-1 (BEI Resources NR-53742), SARS-CoV-2 Delta strain (Addgene plasmid #155130), SARS-CoV-2 Omicron BA.1 (Addgene plasmid #180375), SARS-CoV-2 Arcturus XBB.1.16 (Addgene plasmid #201189), or SARS-CoV-1WT (Addgene plasmid #170447). ..

Expressing:

Article Title: CRISPR with Transcriptional Readout reveals influenza transcription is modulated by NELF and can precipitate an interferon response
Article Snippet: .. Lentivirus expressing codon-optimized NELF-B was generated by seeding 4x105 HEK293T cells in a 6-well plate with D10, followed by transfection with 1 μg DNA from a plasmid encoding codon-optimized NELFB (sequence provided in Dataset S11), and 1 μg DNA consisting of equal amounts of the lentiviral helper plasmids HDM-Hgpm2 (Addgene #204152), HDM-tat1b (Addgene #204154), pRC-CMV-Rev1 (Addgene #164443), and HDM_VSV_G (Addgene #204156). ..

Generated:

Article Title: CRISPR with Transcriptional Readout reveals influenza transcription is modulated by NELF and can precipitate an interferon response
Article Snippet: .. Lentivirus expressing codon-optimized NELF-B was generated by seeding 4x105 HEK293T cells in a 6-well plate with D10, followed by transfection with 1 μg DNA from a plasmid encoding codon-optimized NELFB (sequence provided in Dataset S11), and 1 μg DNA consisting of equal amounts of the lentiviral helper plasmids HDM-Hgpm2 (Addgene #204152), HDM-tat1b (Addgene #204154), pRC-CMV-Rev1 (Addgene #164443), and HDM_VSV_G (Addgene #204156). ..

Sequencing:

Article Title: CRISPR with Transcriptional Readout reveals influenza transcription is modulated by NELF and can precipitate an interferon response
Article Snippet: .. Lentivirus expressing codon-optimized NELF-B was generated by seeding 4x105 HEK293T cells in a 6-well plate with D10, followed by transfection with 1 μg DNA from a plasmid encoding codon-optimized NELFB (sequence provided in Dataset S11), and 1 μg DNA consisting of equal amounts of the lentiviral helper plasmids HDM-Hgpm2 (Addgene #204152), HDM-tat1b (Addgene #204154), pRC-CMV-Rev1 (Addgene #164443), and HDM_VSV_G (Addgene #204156). ..



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